TP53(R175H) mono

[100 µL]

Catalog Number:  CR-M26072

Gene Symbol:  TP53(R175H)

Description:    Anti TP53(R175H) Mouse Monoclonal Antibody

Background:   TP53 belongs to the p53 proteins family. It is ubiquitously expressed, acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are a cause of esophageal cancer (ESCR), lung cancer (LNCR), adrenocortical carcinoma (ADCC), choroid plexus papilloma (CPLPA) and so on.

Immunogen:   A synthetic peptide from the internal region of TP53 which includes the mutation of R175H, human origin

Applications:   ELISA, WB, IHC

Recommended Dilutions:  

ELISA: 1:1000-1:5000

WB: 1:1000-1:1000

IHC: 1:50-1:100

Concentration:    1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:  

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Anti TP53(R175H) antibody recognizes TP53(R175H) of vertebrates.

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant TP53(R175H) and wildtype proteins. Purified His-tagged TP53(R175H) protein(Cat.#CR-P10207) and wildtype protein(Cat.#CR-P10202) were blotted with Anti TP53(R175H) monoclonal antibody(Cat.# CR-M26072).

Immunofluorescence of cells expressing TP53 proteins with anti TP53(R175H) antibody. HEK293T cells were transfected with pCDNA3-GFP TP53(WT) plasmid (left column) or pCDNA3-GFP TP53(R175H) plasmid (right column), then fixed and stained with anti- TP53(R175H) monoclonal antibody (Cat. # CR-M26072).