EGFR(vIII)

[100 µL]

Catalog Number:  CR-M26176

Gene Symbol:  EGFR/HER1/ERBB1

Description:   Anti EGFR(vIII) Mouse Monoclonal Antibody

Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (NSCLC).

Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of vIII, human origin.

Applications:   ELISA, WB, IF, IHC

Recommended Dilutions:  

ELISA: 1:1000-1:5000

WB: 1:1000-1:5000

IHC: 1:50-1:100

Concentration:   1 mg/ml

Host:   Mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:  

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Recognizes EGFR(vIII), but not wild type EGFR from vertebrates.

Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant EGFR(vIII) and wildtype proteins. Purified His-tagged EGFR(vIII) protein (partial, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-EGFR(vIII) mouse monoclonal antibody (Cat. # CR-M26176).

Immunofluorescence:

Immunofluorescence of cells expressing EGFR proteins with anti EGFR(vIII) antibody. HEK293T cells were transfected with pCDNA3-GFP EGFR(WT) plasmid (left column) or pCDNA3-GFP EGFR(vIII) plasmid (right column), then fixed and stained with anti EGFR(vIII) monoclonal antibody (Cat. #CR-M26176).