[ PROPERTIES ]
Residues: Ala101~Pro330 (Accession # O18739), with two N-terminal Tags, His-tag and T7-tag.
Host: E. coli
Subcellular Location: Secreted, extracellular space, extracellular matrix.
Purity: >95% Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. Predicted isoelectric point: 8.6 Predicted
Molecular Mass: 29.3kDa
Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- APCVFGGTVY QSGESFQSSC KY Q CT C L D GS VG CVP L CSV D VR LPS P DCP F PR RV K L PGK C CE EW V C DEP K EHTVVGPALAAYRPEDTFGP DPTMIRANCL VQTTEWSACS KTCGMGISTR VTNDNAFCRL EKQSRLCMVR PCEADLEENI KKGKKCIRTP KISKPIKFEL SGCTSMKTYR AKFCGVCTDG RCCTPHRTTT LPVEFKCPDG EVMKKSMMFI KTCACHYNCP