酶联免疫试剂盒用于定量检测血清、血浆中巨细胞病毒。
PRINCIPLE OF THE TEST Purified CMV antigen is coated on the surface of microwells. Diluted patient serum is added to the wells, and the CMV IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. HRPconjugate is added, which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM specific-antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.