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兔抗内囊体cyt b6/f复合体细胞色素f多克隆抗体

￥5175
Agrisera
美国
2021-06-16 23:14
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产品属性
抗体名兔抗内囊体cyt b6/f复合体细胞色素f多克隆抗体
抗体英文名Cyt f|cytochrome f protein ( PetA) of thylakoid Cyt b6/f-complex
浓度见说明书
应用范围western blot (WB)
宿主兔
适应物种植物
标记物无
克隆性多抗/单行
保存条件低温
亚型IgG
规格50 µl
产品说明

供应商：上海经科化学科技有限公司 

服务热线：400-0199-638 


QQ:472482400(上海经科）


微信号：shjkchem 

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兔抗内囊体cyt b6/f复合体细胞色素f多克隆抗体介绍：

货 号：AS06 119

中文名称：兔抗内囊体cyt b6/f复合体细胞色素f多克隆抗体

英文名称：Cyt f|cytochrome f protein ( PetA) of thylakoid Cyt b6/f-complex

应用：western blot (WB)

规格：50 µl

价格：5175元

兔抗内囊体cyt b6/f复合体细胞色素f多克隆抗体简介：

PRODUCT INFORMATION
background

Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.

immunogen

GST fusion to cytochrome f from Chlamydomonas reinhartii P23577

hostrabbit
clonalitypolyclonal
purityserum,
formatlyophilized
quantity50 µl
reconstitutionFor reconstitution add 50 µl of sterile water.
storagestore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applicationswestern blot (WB)
related products

AS08 306 | anti-cytochrome f (with reactivity to cyanobacteria)

Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

additional informationcontains 0.02 % sodium azide as preservative
APPLICATION INFORMATION
recommended dilution1:2000 with alkaline phosphatase, 1: 50 000 with ECL (WB)
expected | apparent MW34 | 31-32 kDa
confirmed reactivityArabidopsis thaliana, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Hordeum vulgare, Nannochloropsis gaditana, Nicotiana tabacum, Physcomitrella patens, Selaginella martensi
predicted reactivityCitrus x limon, green algae
not reactive inno confirmed exceptions from predicted reactivity known in the moment
additional informationreaction with cyanobacteria: Synechocystis 6803 and Synechococcus 7942 possible to obtain on total cell extract when using antibody at 1: 500 and longer exposure time.
selected referencesFerroni et al. (2016). Light acclimation in the lycophyte Selaginella martensii depends on changes in the amount of photosystems and on the flexibility of the light-harvesting complex II antenna association with both photosystems. New Phytol. 2016 Apr 5. doi: 10.1111/nph.13939. Suorsa et al. (2015). Light acclimation involves dynamic re-organisation of the pigment-protein megacomplexes in non-appressed thylakoid domains. Plant J. 2015 Aug 29. doi: 10.1111/tpj.13004. 
Charuvi et al. (2015). Photoprotection Conferred by Changes in Photosynthetic Protein Levels and Organization during Dehydration of a Homoiochlorophyllous Resurrection Plant. Plant Physiol. 2015 Apr;167(4):1554-65. doi: 10.1104/pp.114.255794. 
Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741. 
Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375. 
​

application example 

Chlamydomonas reinhardtii samples are lysed cells, Arabidopsis thaliana samples are prepared from thylakoid membranes; Samples were boiled 1 min in 3%SDS,then 10ug of chlorophylls were loaded in each well of a 12% acrylamide gel. 

Western blot detection using anti-cytochrome f antibodies
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