| 保存条件 | 常温 |
| 规格 | 250次 |
| PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume | |
• Two applications in one kit, one buffer with optimal performance for both applications |
| Technology | Silica-membrane technology |
| Format | Mini spin columns |
| Sample material | < 400 µL PCR reaction mixture |
| Fragment size | 50 bp–approx. 20 kbp |
| Typical recovery | 70–95% |
| A260/A280 | 1.8–1.9 |
| Elution volume | 15–30 µL |
| Preparation time | 10 min/6 preps |
| Binding capacity | 25 µg |
| Applications | |
• Purification of PCR products | |
| • NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as: - nucleotides, primers - enzymes - mineral oil - PCR additives (e.g., salts, betaine, DMSO) - detergents (e.g., Tween 20, Triton X-100) - dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain) | |
• Support protocol for DNA extraction from polyacrylamide gels | |
| • Typical downstream applications: cloning, sequencing, PCR, restriction analysis | |
