Design of an environmentally sensitive fluorescent 8-aza-7-deaza-2′-deoxyadenosine derivative with dual fluorescence for the specific detection of thymine†,10.1039/C3OB41757C

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Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

Design of an environmentally sensitive fluorescent 8-aza-7-deaza-2′-deoxyadenosine derivative with dual fluorescence for the specific detection of thymine†

Azusa Suzuki,Nobukatsu Nemoto,Isao Saito,Yoshio Saito

Organic & Biomolecular Chemistry Pub Date : 10/16/2013 00:00:00 , DOI:10.1039/C3OB41757C

Abstract

A novel environmentally sensitive fluorescent (ESF) purine nucleoside, ^cnaA, was synthesized and its photophysical properties were investigated. The base-modified fluorescent nucleoside ^cnaA exhibited remarkable solvatochromicity and environmentally sensitive dual fluorescence. By using ^cnaA, we have developed highly thymine (T) selective fluorescent DNA probes that can sense the corresponding T in a target DNA/RNA sequence, as measured by a distinct change in emission wavelength.