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A FRET-based rhodamine–benzimidazole conjugate as a Cu2+-selective colorimetric and ratiometric fluorescence probe that functions as a cytoplasm marker†
Shyamaprosad Goswami,Annada C. Maity,Anup Kumar Maity,Avijit Kumar Das,Partha Saha
RSC Advances Pub Date : 11/19/2013 00:00:00 , DOI:10.1039/C3RA46280C
Abstract

On the basis of fluorescence resonance energy transfer (FRET) from benzimidazole to a rhodamine moiety, a rhodamine–benzimidazole conjugate (RBC) ratiometric fluorescent probe has been designed and synthesized. The RBC selectively binds to Cu2+, showing visually observable changes in absorption and emission behavior, and demonstrates an effective intracellular Cu2+ imaging ability, allowing it to function as a cytoplasm marker.

Graphical abstract: A FRET-based rhodamine–benzimidazole conjugate as a Cu2+-selective colorimetric and ratiometric fluorescence probe that functions as a cytoplasm marker
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