A hexa(choloyl) conjugate bearing a rigid p-phenylenediamine linker was synthesized and shown to produce dimer-based pores in POPC bilayers. This is in sharp contrast to a previous finding that its octa(choloyl) analog formed monomer-based pores, and supplies a fine line between unimolecular and bimolecular pores formed from poly(choloyl) conjugates of this type.