In this study, we used the “cell-SELEX” strategy to generate four new aptamers with high binding affinity for Huh7.5.1. and HepG2 cells, as indicated by Kd values in the low nanomolar range, as well as high specificity compared to L-02 normal human liver cells. Moreover, the aptamers could distinguish human liver tumour tissues from normal tissues. Incorporation of the aptamer into probes permitted activation by target liver cancer cells to yield enhanced fluorescence and the detection of 103 orders of target liver cancer cells. The newly generated aptamers have great potential in early cancer diagnosis.
