Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GTBP1 with in situ product removal†,10.1039/C7RA02367G

热搜：

Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GTBP1 with in situ product removal†

Xuejun He,Bo Fan

RSC Advances Pub Date : 04/26/2017 00:00:00 , DOI:10.1039/C7RA02367G

Abstract

A glycosyltransferase GT_BP1 from Bacillus pumilus BF1 was isolated. Efficient production of ponasterone A was achieved by the recombinant E. coli/gt_BP1 in a biphasic system with a molar yield of 92.7%. This in situ product removal provided the “driving force” for shifting the reaction equilibrium towards the synthesis of the product.