A highly selective probe for UDP-glucuronosyltransferase 2B7 (UGT2B7) in human microsomes: isoform specificity, kinetic characterization, and applications†,10.1039/C4RA09819F

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Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

A highly selective probe for UDP-glucuronosyltransferase 2B7 (UGT2B7) in human microsomes: isoform specificity, kinetic characterization, and applications†

Xiang-Ge Tian,Chao Wang,Guang-Bo Ge,Chun-Zhi Ai,James Y. Hong,Yong-Xv Cai,Xiao-Kui Huo,Jie Hou,Ke-Xin Liu,Hong-Zhi Sun

RSC Advances Pub Date : 11/27/2014 00:00:00 , DOI:10.1039/C4RA09819F

Abstract

3-Epideacetycinobufagin (EDCB) was found to be a highly isoform-specific probe substrate for 3-glucuronidation mediated by UDP-glucuronosyltransferase 2B7 (UGT2B7). The probe reaction was well-characterized, and our results strongly suggest that EDCB can be used specially to measure the catalytic activity of UGT2B7 in biological samples.