In recent years, the concept of nanoparticles being used as an intelligent drug carrier has gained great attention. In this paper, the formulation of a liposome delivery system loaded with a novel benzothiazole-containing cisplatin derivative (CJM-Pt) was carried out. The particle size distributions were determined using dynamic light scattering, and the prepared liposomes showed a suitable size of around 98 nm. Stability studies showed that the CJM-Pt loaded liposomes were stable at 4 째C for more than four weeks. Investigation of triggered release indicated that the release performance of the prepared liposomes was controllable and the releasing effect was remarkable under low pH (<pH 6.8) and high temperature (>42 째C). To test the suitability of the chosen formulation, CJM-Pt loaded liposomes were investigated against several tumor cell lines: MGC-803, SGC-7901, MCF-7 and MDA-MB-231. Furthermore, the cell cycle arrest was examined. The CJM-Pt loaded liposomes have the potential to be applied in drug delivery systems (DDS) for breast cancer therapy.