Fluorescent probe for highly selective and sensitive detection of hydrogen sulfide in living cells and cardiac tissues†
Bifeng Chen,Wei Li,Cong Lv,Manman Zhao,Hongwei Jin,Hongfang Jin,Junbao Du,Liangren Zhang,Xinjing Tang
Abstract
A coumarin-based fluorescence chemoprobe was developed and evaluated for the selective and sensitive detection of hydrogen sulfide in degassed PBS buffers and fetal bovine serum. Fluorescence detection of hydrogen sulfide in living cells was also successfully achieved using two-photon confocal fluorescence imaging. Further in situ visualization of endogenous H2S was realized in cardiac tissues of normal rats and atherosclerosis (AS) rats.
