960化工网
A mitochondria-targeting ratiometric fluorescent probe for the detection of hypochlorite based on the FRET strategy†
Shi-Li Shen,Xiao-Fan Zhang,Yan-Qing Ge,Yan Zhu,Xiao-Qun Cao
RSC Advances Pub Date : 12/05/2017 00:00:00 , DOI:10.1039/C7RA11086C
Abstract

In this study, a ratiometric fluorescent probe (IRP) for OCl was designed based on the fluorescence resonance energy transfer (FRET) platform. The probe was fabricated by integrating an imidazo[1,5-a]pyridine moiety (donor) with a rhodamine moiety (acceptor). We evaluated its properties for recognizing OCl and the results implied that IRP possessed high selectivity, a real-time detection possibility and brilliant photostability. The fluorescence intensity ratios (I575/I467) of IRP displayed desirable OCl-dependent performance and responded linearly to OCl in the concentration range of 0.5–3.5 μM. Also, IRP was applied to image endogenous OCl with a mitochondria-targeting ability, due to the introduction of the quaternized pyridine moiety. Therefore, IRP may be used for further mitochondrial OCl-related studies.

Graphical abstract: A mitochondria-targeting ratiometric fluorescent probe for the detection of hypochlorite based on the FRET strategy
平台客服
平台客服
平台在线客服