A probe of 8,8′-((1Z,1′Z)-(((propane-1,3-diylbis(oxy))bis(2,1-phenylene))bis(azanylylidene))bis(methanylylidene))bis(7-hydroxy-4-methyl-2H-chromen-2-one) (H₂L) was able to recognize Zn²⁺via its turn-on emission at 510 nm in a MeCN–water (9 : 1, v/v) solution in the presence of a large number of biologically important cations with a very low limit of detection (LOD), namely 11 nM, which meant it was about 3500 times more sensitive than the WHO recommended permissible standard for zinc in drinking water (3 ppm). The probe, H₂L, showed solvent polarity sensitive absorption and emission properties and the λ_max showed negative solvatochromism. The composition of the complex, [ZnL], was established by a Job's plot, HR-MS data, and single-crystal X-ray diffraction measurements. The selectivity and sensitivity of the probe, H₂L, was successfully applied to the detection of intracellular Zn²⁺ in human hepato-cellular-carcinoma (HepG2) cells. H₂L is also applicable for the analysis of Zn²⁺ ions in tap water.