A sensitive and rapid technique for high selectivity detection of tetradifon has been developed. This method is based on a new kind of hollow shell molecularly imprinted (MI) polymer. In this method, the new hollow shell MI polymer has bigger specific surface areas compared with the traditional one based on the double-sides hollow microspheres structure. The proposed method has the advantage of wider linear range and lower limit of detection compared with the traditional one. The change in chemiluminescence (CL) intensity is linearly proportional with the concentration of tetradifon in the range 0.1∼12 μg mL⁻¹ and the detection limit has reached 0.0483 μg mL⁻¹. The new MI-CL sensor has been successfully applied to the determination of residual tetradifon in food and the results obtained compare well with those by other methods.