A new enzyme-free quadratic SERS signal amplification approach for circulating microRNA detection in human serum†,10.1039/C5CC06549F

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A new enzyme-free quadratic SERS signal amplification approach for circulating microRNA detection in human serum†

Jing Zheng,Dandan Ma,Muling Shi,Junhui Bai,Yinhui Li,Jinfeng Yang

Chemical Communications Pub Date : 09/10/2015 00:00:00 , DOI:10.1039/C5CC06549F

Abstract

In this communication, we proposed a new enzyme-free quadratic SERS signal amplification approach for the ultrasensitive detection of circulating miRNA in human serum. Combined with miRNA-triggered hybridization chain reaction and Ag⁺-mediated cascade amplification, a limit of miRNA detection as low as 0.3 fM could be achieved. More importantly, our method is suitable for the direct detection of circulating miRNAs in human serum collected from patients with different stages of chronic lymphocytic leukemia (CLL).