A new pyrene based fluorescence probe has been synthesized for fluorogenic detection of Cu2+ in acetonitrile–aqueous media (7 : 3 CH3CN–HEPES buffer, v/v, at pH 7.5) with bioimaging in both prokaryotic (Candida albicans cells) and eukaryotic (Tecoma stans pollen cells) living cells. The anion recognition properties of the sensor have also been studied in acetonitrile by fluorescence methods which show remarkable sensitivity toward fluoride over other anions examined.
