Mass spectrometry enables the discovery of inhibitors of an LPS transport assembly via disruption of protein–protein interactions†,10.1039/D1CC04186J

热搜：

Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

Mass spectrometry enables the discovery of inhibitors of an LPS transport assembly via disruption of protein–protein interactions†

Dante Rotili,Antonello Mai,Carol V. Robinson

Chemical Communications Pub Date : 09/02/2021 00:00:00 , DOI:10.1039/D1CC04186J

Abstract

We developed a native mass spectrometry-based approach to quantify the monomer–dimer equilibrium of the LPS transport protein LptH. We use this method to assess the potency and efficacy of an antimicrobial peptide and small molecule disruptors, obtaining new information on their structure–activity relationships. This approach led to the identification of quinoline-based hit compounds representing the basis for the development of novel LPS transport inhibitors.