Label-free fluorescence light-up detection of T4 polynucleotide kinase activity using the split-to-intact G-quadruplex strategy by ligation-triggered and toehold-mediated strand displacement release†
Lu Zhou,Xiaoqiang Shen,Na Sun,Kewei Wang,Yuanyuan Zhang,Renjun Pei
A label-free, fluorescence light-up detection method for T4 polynucleotide kinase activity has been developed using the split-to-intact G-quadruplex strategy.