In this communication, we report the development of a Ni-based metal–organic framework nanosheet array on Ni foam (Ni-MOF/NF) as an ordered 3D electrode material for the detection of glucose in alkaline media. The Ni-MOF/NF presents remarkable performance, with a long linear range (0.04 to 2 mM) and a low detection limit for glucose concentration up to 85 nM (S/N = 3).