Novel carbon materials derived from biological species attract a great deal of attention due to their characteristic ordered and porous microstructures. In this work, a nanoflower-like CoS-decorated 3D porous carbon skeleton from rose was firstly synthesized through a facial one-pot solvothermal synthetic process and innovatively used to fabricate a high performance nonenzymatic glucose biosensor. The nanoflower-like structure of CoS provided abundant highly electrocatalytic active sites and the carbon skeleton of rose featured plenty of interconnected channels, which could enhance mass diffusion and electron transfer. The prepared sensor exhibited good electrocatalytic performance toward glucose with a linear range from 10 μM to 960 μM, a low detection limit of 2 μM and a high sensitivity of 697 μA mM⁻¹ cm⁻² at the potential of +0.45 V. Overall, this work highlights the great promise offered by this novel nanocomposite material in terms of its application in nonenzymatic sensing.