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Metal free, “click and click–click” conjugation of ribonucleosides and 2′-OMe oligoribonucleotides on the solid phase†
Ishwar Singh,Frances Heaney
Organic & Biomolecular Chemistry Pub Date : 11/26/2009 00:00:00 , DOI:10.1039/B918463E
Abstract

A fast and practical metal free conjugation of ribonucleosides and 2′-OMe 4-mer oligoribonucleotides has been accomplished by a nitrile oxide alkyne click cycloaddition reaction on the solid-phase, the methodology is suited to modification at either, or both, the 3′- or the 5′-terminus of the oligoribonucleotide substrate.

Graphical abstract: Metal free, “click and click–click” conjugation of ribonucleosides and 2′-OMe oligoribonucleotides on the solid phase
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