A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems†,10.1039/D0RA02382E

热搜：

Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems†

Codruţa C. Popescu,Lia-Maria Cucos,Anca G. Coman,Antonio Radoi,Anca Paun,Niculina D. Hădade,Arnaud Gautier,Costin-Ioan Popescu,Mihaela Matache

RSC Advances Pub Date : 06/23/2020 00:00:00 , DOI:10.1039/D0RA02382E

Abstract

We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells.