Here we have studied the complexation of naringin with Al(III) under physiological conditions (i.e., at 37 °C and in 0.16 mol L⁻¹ NaCl). Solubility and protonation constants of the ligand were first determined in order to evaluate the competition of naringin for the Al(III) and H⁺ ions. Speciation analysis made by potentiometric titrations and supported by UV data shows that a complexation occurs at a 1 : 1 ligand-to-Al(III) stoichiometric ratio. Notably, these data combined with mass spectrometry results indicate that the complexation site is located on the β-hydroxy ketone moiety.