We have demonstrated an efficient, selective and environmentally benign heterogeneous catalyst (K-OMS-2) for aerobic oxidation of 5-HMF to 2,5-DFF. In addition, a combination of Fe3O4-SBA-SO3H and K-OMS-2 successfully catalyzed direct synthesis of 2,5-DFF from fructose via acid-catalyzed dehydration and successive aerobic oxidation in one-pot reaction.