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Multiple turnovers of DNAzyme for amplified detection of ATP and reduced thiol in cell homogenates†
Yingshu Guo,Jia liu,Guangxu Yang,Xiaofeng Sun,Hong-Yuan Chen,Jing-Juan Xu
Chemical Communications Pub Date : 11/20/2014 00:00:00 , DOI:10.1039/C4CC08428D
Abstract

A conveniently amplified DNAzyme-based fluorescence strategy was designed for highly sensitive detection of ATP or reduced thiol based on the introduction of an ATP aptamer or a disulfide bond in the bioconjugates of magnetic nanoparticles (MNP) and polystyrene microsphere–DNAzyme complexes (PSM–DNAzyme).

Graphical abstract: Multiple turnovers of DNAzyme for amplified detection of ATP and reduced thiol in cell homogenates
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