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Potassium-induced G-quadruplex DNAzyme as a chemiluminescent sensing platform for highly selective detection of K+
Hong Wang,Dong Mei Wang,Ming Xuan Gao,Jian Wang
Analytical Methods Pub Date : 07/09/2014 00:00:00 , DOI:10.1039/C4AY01411A
Abstract

A simple and highly selective chemiluminescence (CL) detection method for K+ was developed based on a K+-stabilized G-qaudruplex DNAzyme, which catalyzes a luminol-H2O2 reaction system. Herein, a G-quadruplex DNAzyme stemming from a common guanine-rich DNA sequence named PS2.M is introduced as a catalyst. Upon the addition of K+, PS2.M is induced to fold into G-quadruplex as a cofactor binding with hemin, effectively catalyzing the redox reaction of luminol-H2O2. The reaction generates a CL emission and allows the DNAzyme to show a good horseradish peroxidase (HRP) mimicking-enzyme activity. The intensity of CL shows a linear dependence on the concentration of K+ within the range of 2–120 μM with a limit of detection (3σ) of 1.66 μM, giving a vital clue to quantify K+ content in urine samples. This strategy firstly opens up CL as an effective and facile approach to detect K+ with high selectivity.

Graphical abstract: Potassium-induced G-quadruplex DNAzyme as a chemiluminescent sensing platform for highly selective detection of K+
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