Cytochrome P450 RhF displays a high degree of substrate promiscuity, mediating a range of O-dealkylations, aromatic hydroxylations, epoxidations and asymmetric sulfoxidations. The self-sufficient nature of this CYP coupled with its ability to catalyse the oxidation of a wide range of functional groups highlights this enzyme as an excellent starting template for directed evolution and promising alternate to P450 BM3.