Analytical methods for quantitative enantioselective measurements are highly desirable in the life sciences. Existing technologies have disadvantages such as limited temporal resolution, the need for molecular labeling, or high experimental complexity. To overcome these limitations, this work presents a method based on conventional Raman spectroscopy. A systematic investigation of the key parameters is carried out. It is demonstrated that their careful choice provides an opportunity for enantioselective and quantitative analysis of enantiopure systems as well as enantiomer mixtures.