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Rational redesign of Candida antarcticalipase B for the ring opening polymerization of d,d-lactide†
Mohamad Takwa,Marianne Wittrup Larsen,Karl Hult,Mats Martinelle
Chemical Communications Pub Date : 05/23/2011 00:00:00 , DOI:10.1039/C1CC10865D
Abstract

Based on molecular modelling, the enzymeCandida antarcticalipase B was redesigned as a catalyst for the ring opening polymerization of D,D-lactide. Two mutants with 90-fold increased activity as compared to the wild-type enzyme were created. In a preparative synthesis of poly(D,D-lactide) the mutants greatly improved the rate and the degree of polymerization.

Graphical abstract: Rational redesign of Candida antarcticalipase B for the ring opening polymerization of d,d-lactide
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