A three-way junction structure-based isothermal exponential amplification strategy for sensitive detection of 3′-terminal 2′-O-methylated plant microRNA†,10.1039/C6CC08726D

热搜：

Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

A three-way junction structure-based isothermal exponential amplification strategy for sensitive detection of 3′-terminal 2′-O-methylated plant microRNA†

Xiangdong Wang,Hui Wang,Chenghui Liu,Honghong Wang,Zhengping Li

Chemical Communications Pub Date : 12/23/2016 00:00:00 , DOI:10.1039/C6CC08726D

Abstract

Plant microRNAs (miRNAs) differ from animal miRNAs in that they are 2′-O-methylated at the 3′ end nucleotide which limits the ability of plant miRNAs to serve as primers for polymerization reactions. Herein, we have rationally designed a three-way junction structure-based isothermal exponential amplification reaction (3WJ-EXPAR) which is well-suited for sensitive detection of 3′-terminal 2′-O-methylated miRNAs.