Three novel conjugated polymers based on 2,4,6-triphenylpyridine moieties were synthesized by a Pd-catalyzed Sonogashira coupling reaction. The three polymers P-1, P-2 and P-3 showed strong fluorescence emission with large Stokes' shifts and high quantum yields. We also performed a theoretical calculation analysis to predict the electrochemical and optical properties of the conjugated polymers. The results indicated that although the calculated energy levels were higher than those determined by experiments, the trends of P-1, P-2 and P-3 in the band gaps were in good agreement with the ones obtained by UV-vis and CV measurements of the polymers.