The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases†,10.1039/D0CC03721D

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Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases†

Weihua Xu,Chenggong Hui,Jian Xu,Meilan Huang,Xianfu Lin,Qi Wu

Chemical Communications Pub Date : 07/08/2020 00:00:00 , DOI:10.1039/D0CC03721D

Abstract

The mutagenesis of a “second sphere” switch residue of CHMO_Acineto could control its enantio- and regiopreference. Replacing phenylalanine (F) at position 277 of CHMO_Acineto into larger tryptophan (W) enabled a significant enhancement of enantio- or regioselectivity toward structurally diverse substrates, moreover, a complete reversal of enantio- or regiopreference was realized by mutating F277 into a range of smaller amino acids (A/C/D/E/G/H/I/K/L/M/N/P/Q/R/S/T/V).