Synthesis and structural studies of S-type/N-type-locked/frozen nucleoside analogues and their incorporation in RNA-selective, nuclease resistant 2′–5′ linked oligonucleotides†,10.1039/C2OB26762D

热搜：

Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

Synthesis and structural studies of S-type/N-type-locked/frozen nucleoside analogues and their incorporation in RNA-selective, nuclease resistant 2′–5′ linked oligonucleotides†

Namrata Erande,Anita D. Gunjal,Moneesha Fernandes,Rajesh Gonnade,Vaijayanti A. Kumar

Organic & Biomolecular Chemistry Pub Date : 11/07/2012 00:00:00 , DOI:10.1039/C2OB26762D

Abstract

2′-endo locked or frozen (S-type)/3′-endo locked or frozen (N-type) nucleoside analogues were synthesized. Conformational analysis based on ³J_HH and NOE measurements is presented which is further confirmed by X-ray crystal structural studies. 2′–5′ isoDNA oligonucleotides (ON) were synthesized using these modified nucleoside analogues and UV-T_m studies of the resultant 2′–5′ isoDNA : RNA duplexes reflect the site- and sequence-dependent effects and confirm that the S-type sugar conformations were preferred over the N-type sugar geometry in such duplexes.