Unexpected stereoselective exchange of straight-chain fatty acyl-CoA α-protons by human α-methylacyl-CoA racemase 1A (P504S)†,10.1039/C002509G

热搜：

Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

Unexpected stereoselective exchange of straight-chain fatty acyl-CoA α-protons by human α-methylacyl-CoA racemase 1A (P504S)†

Fouzia A. Sattar,Daniel J. Darley,Francesco Politano,Timothy J. Woodman,Michael D. Threadgill,Matthew D. Lloyd

Chemical Communications Pub Date : 03/30/2010 00:00:00 , DOI:10.1039/C002509G

Abstract

α-Methylacyl-CoA racemase (AMACR; P504S) catalysed exchange of straight-chain fatty acyl-CoA α-protons. One α-proton was removed in each catalytic cycle, with the pro-S proton preferred. This reaction was most efficient for straight-chain substrates with longer side-chains. 2-Methyldecanoyl-CoA underwent α-proton exchange 3× more efficiently (as judged by K_cat/K_m) than decanoyl-CoA.