In this work, we have demonstrated selective patterning of electrodes coated with N-[3-(trimethoxysilyl)propyl]ethylenediamine (EDAS) stabilised gold nanoparticles (Sil-AuNps) films with Prussian blue (PB) by continuous electrochemical cycling of Sil-AuNps film modified Indium Tin Oxide (ITO) electrode in K₃[Fe(CN₆)] solution. The PB formed on gold nanoparticles (PB@Sil-AuNps) exhibits an electrochemical response similar to the PB formed on a bare ITO surface. However, the high spin iron redox state in PB formed on Sil-AuNps shows almost zero peak to peak separation (ΔE_p) value in contrast to the finite ΔE_p for PB formed on bare ITO. In addition, this selectively patterned PB on Sil-AuNps modified ITO electrode shows characteristic voltammetric responses of both PB and gold in 0.1 M KNO₃ and 0.5 M H₂SO₄ respectively. In order to see the coverage of PB over gold nanoparticles, ITO electrode surface was characterised using scanning electron microscopy (SEM) and energy dispersive X-rays analysis (EDX) techniques. Selective patterning of redox mediator on noble metal nanoparticles results in lower film resistivity, which favours high electrocatalytic activity towards hydrogen peroxide electro-reduction. The sensitivity of this modified electrode towards H₂O₂ is 3.4 nA nM⁻¹.