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Two-photon active polymeric nanoparticles for high contrast in vitro imaging†
Lian Li,Lynne A. Samuelson,Dhimiter Bello
RSC Advances Pub Date : 11/14/2013 00:00:00 , DOI:10.1039/C3RA44576C
Abstract

Two water dispersible and two-photon (TP) active polymer nanoparticles were synthesized from poly[2-(3-thienyl) ethanol n-butoxy carbonyl methyl-urethane] and poly(3,3′′′-didodecyl quarter thiophene) polymers using a simple miniemulsion technique. Strong TP fluorescence was observed from each of these nanoparticles using a femtosecond laser operated at 800 nm. Interestingly, these polymeric nanoparticles were found to be non-toxic in dose dependent cell viability studies for 24 h nanoparticle incubation time period. TP microscopic imaging and Z-stacking confirmed cellular internalization of TP active nanoparticles by human macrophage THP-1 cells. These fluorescent polymer nanoparticles could be suitable candidates for in vitro TP bioimaging.

Graphical abstract: Two-photon active polymeric nanoparticles for high contrast in vitro imaging
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