Switching glycosyltransferase UGTBL1 regioselectivity toward polydatin synthesis using a semi-rational design†,10.1039/C8OB00376A

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Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

Switching glycosyltransferase UGTBL1 regioselectivity toward polydatin synthesis using a semi-rational design†

Bo Fan,Wenxin Dong,Tianyi Chen

Organic & Biomolecular Chemistry Pub Date : 03/15/2018 00:00:00 , DOI:10.1039/C8OB00376A

Abstract

The 62nd residue of glycosyltransferase UGT_BL1 was identified as a “hotspot” for glycosylation at 3-OH of resveratrol. Via semi-rational design including structure-guided alanine scanning and saturation mutations, the mutation I62G significantly switched the regioselectivity from 4′-OH to 3-OH of resveratrol and mainly produced polydatin (87.7%), a therapeutic natural product.