Aggregation-induced emission (AIE) amphiphilic block copolymers were developed based on the living ring-opening metathesis polymerization for the first time. By virtue of the block copolymer self-assembly in selective solvents, water soluble fluorescent nano-objects were then prepared with various structures including spherical micelles, cylindrical micelles, and vesicles. This method represents a facile and efficient way to prepare the well-defined AIE polymers and the varied fluorescent nano-objects with controlled structures and functionalities.