Transdermal delivery of small interfering RNAs with topically applied mesoporous silica nanoparticles for facile skin cancer treatment†,10.1039/C9NR06303J

热搜：

Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

Transdermal delivery of small interfering RNAs with topically applied mesoporous silica nanoparticles for facile skin cancer treatment†

Chenghao Liu,Myo Myo Swe Oo,Christian Wiraja,Melissa Hui Yen Teo,Mengjia Zheng

Nanoscale Pub Date : 09/03/2019 00:00:00 , DOI:10.1039/C9NR06303J

Abstract

Small interfering RNA (siRNA) is a promising tool for the treatment of skin disorders including skin squamous cell carcinoma (SCC). This article develops a topical formulation for the transdermal delivery of siRNA. The formulation is built on mesoporous silica nanoparticles (MSNPs) with a loading capacity of 1.4 μg of oligonucleotide per mg of MSNPs. Cell experiments are employed to study the functionality of the formulation including the cellular uptake, the qualitative and quantitative detection of specific gene biomarkers. The clinical potential of this system is examined by topically delivering siRNA targeting TGFβR-1 (TGFβR-1) to the SCC in a mouse xenograft model. In comparison to the controls, MSNPs containing TGFβR-1 siRNA show a 2-fold suppression of TGFβR-1.