In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol–ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG410K-SH). Biotinylated HL-60 cells were selectively adhered onto an avidin-patterned surface with high viability.