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Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays†
Yasuhiko Iwasaki,Tatsuya Ota
Chemical Communications Pub Date : 08/19/2011 00:00:00 , DOI:10.1039/C1CC12948A
Abstract

In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol–ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG410K-SH). Biotinylated HL-60 cells were selectively adhered onto an avidin-patterned surface with high viability.

Graphical abstract: Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays
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