An anionic conjugated polymer as a multi-action sensor for the sensitive detection of Cu2+ and PPi, real-time ALP assaying and cell imaging†,10.1039/C5AN00905G

热搜：

Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

An anionic conjugated polymer as a multi-action sensor for the sensitive detection of Cu2+ and PPi, real-time ALP assaying and cell imaging†

Akhtar H. Malik,Sameer Hussain,Arvin Sain Tanwar,Sourav Layek,Vishal Trivedi,Parameswar K. Iyer

Analyst Pub Date : 05/14/2015 00:00:00 , DOI:10.1039/C5AN00905G

Abstract

A Cu²⁺ ensemble polyfluorene derivative, poly[5,5′-(((9H-fluorene-9,9-diyl)bis(hexane-6,1-diyl))bis(oxy))diisophthalate] sodium salt (PFT), displays unprecedented selectivity for PPi (LOD = 2.26 ppb) in aqueous solution as well as in random urine samples at physiological pH vis-a-vis monitoring ALP activity. Furthermore, intracellular imaging of Cu²⁺ and PPi in mouse macrophage (J774A.1) and human breast cancer cells (MDA-MB231) was achieved to confirm the viability of PFT in biological systems.