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Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging†
Setsuko Tsuboi,Takao Sakata,Hidehiro Yasuda
Chemical Communications Pub Date : 08/02/2017 00:00:00 , DOI:10.1039/C7CC04966H
Abstract

A facile method for the preparation of antibody–quantum dot (QD) conjugates using the immunoglobulin binding (B1) domain of protein G is presented. The utility of antibody–QD conjugates using the B1 domain is demonstrated for fluorescence imaging of breast tumor cells in vitro and in vivo.

Graphical abstract: Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging
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