On-point detection of GM rice in 20 minutes with pullulan as CPA acceleration additive†,10.1039/C4AY02427C

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Lactoyl-CoA 全氟丁基戊烷白叶藤碱全氟己基辛烷新白叶藤碱壳寡糖特色专题-生物多肽

On-point detection of GM rice in 20 minutes with pullulan as CPA acceleration additive†

Rui Wang,Jian Wu,Fang Zhang,Liu Wang,Feng Ji

Analytical Methods Pub Date : 10/22/2014 00:00:00 , DOI:10.1039/C4AY02427C

Abstract

1% (w/v) concentration of pullulan as a cross-priming isothermal amplification (CPA) reaction additive has a great acceleration effect on nucleic acid amplification. Using this property of pullulan, on-point specific detection of nucleic acid by lateral-flow dipstick (LFD) within 20 minutes was achieved with initial DNA template of 750 copies.