A detailed analysis of the ECL interaction between luminol and tris(2,2′-bipyridyl)dichlororuthenium(II) (Ru(bpy)32+) is required before using them in ECL systems for multianalyte detection purposes. Spectro-electrochemiluminescence demonstrates that not only must the emission properties be considered, but also their additional optical characteristics are involved in the explanation of the interaction mechanism between these luminophores.
