The control of biofouling is crucial in the membrane separation process. In the present study, graphene oxide-silver (GO-Ag) nanocomposite was fabricated and incorporated into polycaprolactone (PCL) membrane for the first time to increase its antibacterial properties and reduce its biofouling. Characterization of GO-Ag nanocomposite was done by X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier-transform infrared (FTIR) spectroscopy. Also, The PCL/GO-Ag membranes were characterized by XRD, attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), porosity determination, water contact angle measurement, and tensile test. Furthermore, pure water flux (PWF) and bovine serum albumin (BSA) rejection of the membranes were evaluated. Also, the antibacterial activity of membranes was investigated by diffusion inhibition zone test and plate count of colony-forming units.