T lymphocytes have a primary role in both health and disease. Extracellular and intracellular signals determine whether a T-cell activates different cells, divides, or begins apoptosis. The reaction–diffusion process of Ca2+ ions is critical for the initiation, sustenance, and termination of the immunological function of T cell. A nonlinear spatio-temporal dynamics of Ca2+ in T cells is modeled incorporating parameters Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump, Ryanodine receptor, source amplitude, and buffers. A numerical meshless approach using multiquadric radial basis functions (MQRBF), differential quadrature, and Runge–Kutta method is developed for the solution. The results obtained here give better insights of calcium dynamics in T cells.