In this work, we presented a new tetraphenylethene-derived fluorescent probe TPE-M for Hg²⁺ detection in an aqueous solution. Probe TPE-M is molecularly dissolved in CH₃OH/PBS (20 mM, pH = 7.4) (3 : 7, v/v) mixed solution and is almost non-emissive. Reaction of TPE-M with Hg²⁺ leads to release of an AIE-active precursor 4, and results in a significant fluorescence enhancement. The Hg²⁺ recognition process has some distinct advantages including rapid response, high selectivity and sensitivity, strong anti-interference ability, and a low detection limit (4.16 × 10⁻⁶ M). Moreover, the probe is applicable to detect Hg²⁺ in real food samples such as shrimp, crab and teas, suggesting the practical applicability of TPE-M.